Legionella Field Test Kit
This kit is designed to test for Legionella in risk areas identified by CDC* such as:
- Domestic and industrial hot and cold water systems
- Pools, hot tubs and decorative fountains
- Cooling towers
- Sinks and showers
- Misters, sprinklers, air washers, humidifiers and others
The Legionella Field Test uses Lateral Flow Immunochromatographic Assay (LFICA) technology to detect the presence of cell surface antigens from Legionella bacteria. It uses Legionella antibodies, tagged with red nanoparticles, which bind to any bacteria found in the sample and make them visible on a line on the device. The test works just like a pregnancy test - one line indicates a negative result and two lines indicate a positive result.
*Centers for Disease Control and Prevention
Legionella Field Test Kit
|Bacteria detected||Legionella pneumophila serogroup 1|
|Detection method||Lateral flow immunochromatographic assay|
|Lower limit of detection||As low as 100 CFU/L (0.1 CFU/mL)|
|Specificity||Tested against a wide range of waterborne bacteria, including Acinetobacter calcoaceticus, Aeromonas hydrophila subsp. hydrophila, Bacillus subtilis, Burkholderia cepacia, Citrobacter freundii, Citrobacter koseri, Escherichia coli, Enterobacter cloacae, Klebsiella oxytoca, Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas putida, Pseudomonas
stutzeri, Ralstonia pickettii, Raoultella terrigena, Streptococcus pyogenes, Yersinia ruckeri.
No cross reactions have been observed at concentrations ≤ 109 CFU/L. Staphylococcus aureus has been observed to cross-react with the test at concentrations > 108 CFU/L, which is higher than found in most water samples.
|Organism (≥cfu/mL)||L.p Sg-2,3,8,11,13,14 (1.00E+08)
L.p. Sg-4,5,6,7,9,10,15 (1.00E+07)
L.p. Sg-12 (8.00E+06)
The Legionella pneumophila SG1 test has been shown to produce weak positive results with other legionella pneumophila serogroups at the cfu/mL stated in the above table.
|Test volume||100 μL|
|Suggested sample volume||250 mL|
|Sample parameters||Sample should filter in less than 10 minutes.|
|Biocides||The product has been tested with a range of common biocides and biodispersants used in
Legionella control, at their normal maximum operating concentration. Active ingredients
tested include: glutaraldehyde, dodecyl-dimethyl-ammonium chloride, isolthazoles,
dibromonitrilopropionamide, ionic and non-ionic surfactants. The Test gives a false positive if used with polymeric biguanide or THPS. It should not be used to test such systems.
|Operator time||5–10 minutes|
|Test time||25 minutes|
|Shelf life||18 months from date of manufacture, when stored at room temperature (<30°C) in original
packaging. All test strips are hermetically foil-wrapped and with a printed expiration date.